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mouse pancreatic islet β cell line nit 1 (ATCC)

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ATCC mouse pancreatic islet β cell line nit 1
Mouse Pancreatic Islet β Cell Line Nit 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+pancreatic+%CE%B2+cell+line+nit+1+cells/NIT-1/pmc12757666-57-1-10
Average 95 stars, based on 217 article reviews

mouse pancreatic islet β cell line nit 1 - by Bioz Stars, 2026-10

95/100 stars

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Article Title: Adrenomedullin and its receptors are expressed in mouse pancreatic β-cells and suppresses insulin synthesis and secretion.
Article Snippet: .. The mouse pancreatic β-cell line NIT-1 cells (ATCC CRL-2055, Manassas, VA, USA) were cultured in Ham’ F12K medium supplemented with 2mM L-glutamine (Corning Cellgro, Manassas, VA, USA), 1.5g/L sodium bicarbonate (SIGMA, St. Louis, MO, USA), 10% heatinactivated dialyzed fetal bovine serum (GIBCO, Gaithersburg, MD, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Corning Cellgro, Manassas, VA, USA) in a 5% CO2 atmosphere at 37 ̊ C. NIT-1 cells (3 x 10 cells/well) were seeded in 6-well-plates and cultured for 3 days prior to the treatments. .. After a 30-min preincubation, the cells were treated with increasing doses of glucose (Sigma-Aldrich, St. Louis, MO, USA) in the presence or absence of ADM (10−9 M, American Peptide Co., Inc. Sunnyvale, CA, USA) for 24 hours.

Article Title: Adrenomedullin and its receptors are expressed in mouse pancreatic β-cells and suppresses insulin synthesis and secretion
Article Snippet: .. The mouse pancreatic β-cell line NIT-1 cells (ATCC CRL-2055, Manassas, VA, USA) were cultured in Ham’ F12K medium supplemented with 2mM L-glutamine (Corning Cellgro, Manassas, VA, USA), 1.5g/L sodium bicarbonate (SIGMA, St. Louis, MO, USA), 10% heat-inactivated dialyzed fetal bovine serum (GIBCO, Gaithersburg, MD, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Corning Cellgro, Manassas, VA, USA) in a 5% CO 2 atmosphere at 37° C. NIT-1 cells (3 x 10 cells/well) were seeded in 6-well-plates and cultured for 3 days prior to the treatments. .. After a 30-min preincubation, the cells were treated with increasing doses of glucose (Sigma-Aldrich, St. Louis, MO, USA) in the presence or absence of ADM (10 −9 M, American Peptide Co., Inc. Sunnyvale, CA, USA) for 24 hours.

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mouse pancreatic islet β cell line nit 1 (ATCC)

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